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Illumination and contrast

Draft outline

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For users (biologists) choosing how to light a sample.

Brightfield​

  • Transmitted light; when it's enough; the role of a diffusor for even illumination.

Oblique / darkfield and the LED matrix​

  • What patterned illumination from the LED matrix enables.

Differential phase contrast (DPC)​

  • Turning transparent (phase) samples into contrast, computationally, from opposing illumination halves. When to prefer it over brightfield.

Fluorescence​

  • Labels/dyes and matching laser lines; contrast from emission, not transmission.
TODO

Notion sources: FAT Part 3/7/8 (Matrix LED, DPC), the shadow/diffusor investigation in FAT part 7. Consider a small figure comparing brightfield vs. DPC vs. fluorescence.